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Tuotteen numero:
97964044
50X TAE Buffer, 100 mL
Tuotteen numero:
97964044
€ 42
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Tuotu myymälästä paikasta USA
Tee tilaus nyt, niin saat tuotteen noin Keskiviikko, Heinäkuu 08
Suojattu tapahtuma
Ubuy tekee kovasti töitä turvallisuutesi ja yksityisyytesi suojaamiseksi. Kehittynyt maksun turvallisuusjärjestelmämme varmistaa luottamuksellisuuden salaamalla tietosi siirron aikana AES (Advanced Encryption Standards)- ja SSL (Secure Socket Layer) -protokollilla. Maksutietosi ovat 100 % turvassa, sillä emme jaa maksutietojasi kolmansien osapuolten myyjille.
Tärkeimmät logistiikkakumppanimme
50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.
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Mikä erottuu joukosta
Concentrated Solution
This 50X TAE buffer provides a highly concentrated formulation, ensuring ease of use and longer shelf life, making it ideal for high-throughput laboratories and educational institutions.
Monipuoliset sovellukset
Designed for various molecular biology techniques, this buffer ensures optimal performance for DNA electrophoresis and other applications, effectively meeting diverse research needs in a single product.
Precise Composition
Formulated with high purity components, this TAE buffer guarantees reproducibility and reliability in experiments, catering to both novice and expert researchers who demand consistent results.
Tuotteen yksityiskohdat
| Tuotteen paino | 0.25 lbs (110 grams) |
Kenelle sopii?
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Molecular Biologists
Ideal for electrophoresis applications, aiding in the separation and analysis of nucleic acids in a lab environment.
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Academic Researchers
Perfect for students and researchers conducting experiments that require buffer solutions in their molecular biology studies.
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DNA & RNA Studies
Essential for protocols involving DNA and RNA handling, ensuring stability and integrity during electrophoresis experiments.
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Clinical Laboratories
Not suitable for clinical environments where strict regulatory compliance and high precision are required in diagnostics.
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Undergraduate Courses
Beginner students may find ease of use with simpler buffers; TAE may be too complex for introductory labs.
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Non-Biological Users
Individuals not engaged in biological research or laboratory work would not need this specialized buffer solution.
TUOTEKUVAUS.
50X TAE Buffer, 100 mL
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Asiakkaiden kysymykset ja vastaukset
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kysymys:
What is 50X TAE Buffer used for?
vastaus: 50X TAE Buffer is primarily used in molecular biology for the electrophoresis of nucleic acids. This buffer maintains a stable pH during the separation of DNA and RNA and provides essential ions that help in the migration of nucleic acids through gels. When diluted to 1X concentration, it is optimal for running agarose gels, making it crucial in applications such as gel electrophoresis and DNA fragment analysis. -
kysymys:
How do I dilute 50X TAE Buffer for use?
vastaus: To use 50X TAE Buffer, it needs to be diluted to 1X concentration before application. This is achieved by mixing 1 part of the 50X solution with 49 parts of distilled water. For example, if you need 100 mL of 1X TAE Buffer, combine 2 mL of 50X TAE Buffer with 98 mL of water. This dilution is vital for effective DNA separation and band visualization in gel electrophoresis. -
kysymys:
What is the shelf life of 50X TAE Buffer?
vastaus: The shelf life of 50X TAE Buffer varies depending on storage conditions, but generally, it can remain stable for extended periods if kept in a cool and dry place. To ensure optimal performance, always check for any turbidity, discoloration, or precipitation before use. Proper storage will help maintain its effectiveness for experiments that involve DNA manipulation and analysis, reducing the risk of experimental failure. -
kysymys:
Is 50X TAE Buffer compatible with agarose gel electrophoresis?
vastaus: Yes, 50X TAE Buffer is highly compatible with agarose gel electrophoresis. It provides the necessary ionic environment that stabilizes the DNA while separating it based on size. This buffer is preferred for applications requiring high resolution in DNA fragment size determination. When diluted appropriately to 1X, it ensures effective migration of DNA through the gel matrix, allowing for clear visualization of the bands. -
kysymys:
Can I use 50X TAE Buffer for RNA electrophoresis?
vastaus: Yes, 50X TAE Buffer can be used for RNA electrophoresis, but its use in RNA analysis should be approached with care. It is important to ensure that the RNA samples are not degraded, and the buffer is fresh. RNA is sensitive to degradation; therefore, using 50X TAE Buffer allows better resolution of RNA bands when diluted correctly, promoting accurate electrophoresis results. -
kysymys:
What are the components of 50X TAE Buffer?
vastaus: 50X TAE Buffer consists of Tris base, acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain pH, acetic acid provides the acidic environment, and EDTA chelates divalent metal ions, which can otherwise interfere with DNA and enzymes. The combination creates a stable buffer system essential for nucleic acid studies, providing researchers with optimal conditions for their experiments. -
kysymys:
Is 50X TAE Buffer necessary for DNA gel extraction?
vastaus: 50X TAE Buffer is not strictly necessary for DNA gel extraction but is useful in maintaining the integrity of DNA post-electrophoresis. After running your gel, the buffer can help rehydrate the gel and provide a stable pH environment for further DNA processing. It can also be beneficial when preparing samples for downstream applications, allowing for better recovery of DNA fragments due to its compatibility with various procedures. -
kysymys:
How should I store 50X TAE Buffer?
vastaus: To preserve the stability of 50X TAE Buffer, it should be stored in a cool, dark place at room temperature or refrigerated. Make sure the container is sealed tightly to prevent contamination. Proper storage conditions help prevent degradation or changes in composition, ensuring reliable results during experiments involving nucleic acids, such as PCR preparation or gel electrophoresis. -
kysymys:
What are some common applications for using 50X TAE Buffer?
vastaus: 50X TAE Buffer has several applications, primarily in molecular biology, including gel electrophoresis for DNA and RNA, restriction enzyme digestion, and PCR preparations. It helps facilitate the migration of nucleic acids through gels, aiding in the quantification and analysis of DNA fragments. Researchers often use it for cloning experiments and during the preparation of samples for sequencing, ensuring high-quality results. -
kysymys:
Where can I buy 50X TAE Buffer, 100 mL?
vastaus: You can buy 50X TAE Buffer, 100 mL on Ubuy in Finland. Ubuy provides a convenient online shopping platform where you can find various laboratory supplies, including different concentrations of TAE Buffer, ensuring you get the right product for your research needs.
eCon Lab Supply Buffers Editorial Review
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Plussat
- High purity formula
- Convenient 100 mL size
- Well-suited for various applications
- Easy to store and use
- Luotettava suorituskyky
Miinukset
- Cap can be slightly hard to open.
Tuotteen hintahistoria
Tärkeitä tietoja
- Rajoitukset: Kansainvälisesti toimitettujen tuotteiden kohdalla tulee ottaa huomioon, ettei valmistajan takuu välttämättä ole voimassa, etteivät valmistajan huoltovaihtoehdot välttämättä ole käytettävissä, etteivät tuotteen ohjekirjat, oppaat ja turvallisuusvaroitukset välttämättä ole kohdemaan kielellä, ettei tuotteita (ja niiden oheismateriaalia) ole välttämättä suunniteltu vastaamaan kohdemaan standardeja, teknisiä tietoja ja merkintävaatimuksia ja etteivät tuotteet välttämättä vastaa kohdemaan jännitettä ja muita sähköstandardeja (jolloin ne saattavat tarvittaessa vaatia sovittimen tai muuntimen käyttöä). Vastaanottaja on vastuussa sen varmistamisesta, että tuote voidaan toimittaa kohdemaahan laillisesti. Kun vastaanottaja tekee tilauksen Ubuysta tai sen kumppanuusyhtiöiltä, hän on myös rekisteröity maahantuoja ja hänen tulee noudattaa kaikkia kohdemaan lakeja ja säädöksiä.
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€ 42
Tee tilaus nyt, niin saat tuotteen noin Keskiviikko, Heinäkuu 08
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Suojattu tapahtuma
Ubuy tekee kovasti töitä turvallisuutesi ja yksityisyytesi suojaamiseksi. Kehittynyt maksun turvallisuusjärjestelmämme varmistaa luottamuksellisuuden salaamalla tietosi siirron aikana AES (Advanced Encryption Standards)- ja SSL (Secure Socket Layer) -protokollilla. Maksutietosi ovat 100 % turvassa, sillä emme jaa maksutietojasi kolmansien osapuolten myyjille.
Tärkeimmät logistiikkakumppanimme
Nopein toimitus toiseen maahan
Ominaisuudet ja hyödyt
- Contains 2M Tris, 1M Acetic acid, and 0.05M EDTA for effective nucleic acid electrophoresis.
- Inhibits nucleases to protect DNA integrity during experiments.
- Powers efficient running of both genomic and supercoiled DNA.
- Faster dsDNA run compared to TBE buffer, enhancing experiment efficiency.
- Manufactured under strict quality control in an ISO certified facility.
- Ideal for research purposes, compliant with Good Manufacturing Practices.